Revathi Shanmugam

5 Questions 6 Answers 0 Followers

Questions related from Revathi Shanmugam

Is it necessary to store pH 6.8 and pH 8.8 tris buffer at 4 degrees?

We use to prepare 1.5M tris pH 8.8 and 1 Mtris ph 6.8 for SDS and we keep it at room temperature. After few days I could see there is a increase in pH

25 July 2014 6,363 12 View

I am doing western blotting and the transfer of protein from gel to PVDF membrane is not that muck efficient. How can I trouble shoot it?

Nearlly 5 to 6 times I have done my western blotting, in which not all the protein in my gel is transferred efficiently to PVDF membrane and we use wet transfer method for blotting 100 volts for...

25 July 2014 3,923 9 View

After doing harsh stripping to the PVDF membrane, can we do Ponceau Red staining?

When we add Ponceau Red to the fresh transfer blot we could see the protein transferred to the membrane, but once that membrane was stripped by a harsh method, we could not see the protein bands...

02 July 2014 2,021 3 View

Why are we quantifying the gene of interest say erbb3 with the housekeeping gene? What is the concept behind it?

And can any one explain to me the delta Ct method that is used to quantify the gene of interest?

05 May 2014 9,027 14 View

Why do we use marker for PCR amplified cDNA samples?

Generally to know the molecular weight of the unknown sample we add marker, but in this case of cDNA we know the size of the base pair that we are going to amplify so why do we need the marker?

18 April 2014 7,596 5 View