How can I induce 1,25 Vitamin-D synthesis from skin biopsies in a transwell system, such that the active vitamin-D will have biological effect?

I am trying to replicate results I see in-vitro, with cells cultured with 1,25 dihydroxyvitamin-D (1,25 VitD) but by getting skin biopsies (from healthy donors of surgical procedures) to produce...

22 July 2016 3,960 6 View