Renuka Gupta

3 Questions 3 Answers 0 Followers

Questions related from Renuka Gupta

Lentiviral Transduction of iPSCs?

Hi , I have been trying to transduce my iPSCs with sgRNA , at MOI 5 and 10 and I have had very less efficiency . (3-4%) My Lentiviral supernatant is concentrated. Could some one provide some...

14 July 2023 1,557 4 View

Generation of iPSC dCAS9 lines?

Hi Has anyone generated Stable dCAS9 iPSC cells lines ? What are the methods that you have used? Have you tried selection using a flurophore which can be determined using FACs and sorted...

29 November 2022 6,711 0 View

HiPSC culture cell debris and black dot like bodies?

Hi , I have been working with iPSC cell lines and hESCs for 6 years with Matrigel and mTESR1. I have recently started working with iPSC cells with vitronectin matrix and Stemflex media ....

19 November 2021 324 3 View