Renu Verma

5 Questions 11 Answers 0 Followers

Questions related from Renu Verma

RNA extraction from MTB?

Dear All, I am looking for a kit to extract total RNA from MTB which I will later use for RT-qPCR. My initial sample would be very low and cannot afford to loose RNA during the extraction. Also,...

07 July 2019 8,742 6 View

Can I use ELISA TMB substrate for Western blot?

I am using HRP conjugated antibody.

10 October 2013 6,954 12 View

I want to shear bacterial genomic DNA for PCR. Has anyone tried vortexing?

09 September 2013 1,393 5 View

I received taq DNA polymerase today and it was all stuck to the walls of tube. I gave it a 3 sec spin in centrifuge. Will it affect its activity?

09 September 2013 5,249 4 View

I am getting multiple bands with the gene I amplified 6 months ago.

I amplified a gene from Mycobacterium tuberculosis 6 months ago. I got a single band on the gel corresponding to the gene but now when I amplified the same gene with the same amplification...

06 June 2013 8,171 10 View