Renu Bisht

5 Questions 15 Answers 0 Followers

Questions related from Renu Bisht

Choice of stain for visualization under Confocal microscope for mycobacteria?

Dear All, I have a couple of GFP tagged proteins that I want to study for localization in Mycobacteria. Which stains should be utilized for this purpose (if the cell wall to be stained). Can...

01 January 2020 4,762 2 View

Hi everyone! Does anyone has experience in RNA extraction from mycobacterial cells grown as biofilms (both bead beater based or any other method)?

I am interested in studying the gene expression using Real time PCR and looking for a method that works for isolating RNA from mycobacterium marinum biofilms. Kindly share the protocols Thanks in...

02 February 2019 7,464 9 View

Can anyone tell me the procedure to make static biofilms for visualization under confocal microscope. I want to study the biofilm architecture.?

Biofilm is a fragile structure and can be easily disintegrated, so is there any method to make biofilm slides to study its architecture and morphology?

07 July 2018 5,462 4 View

Hi everyone! Can anyone provide me a protocol for fixation of bacterial cells (mycobacterium smegmatis) for confocal microscopy ?

I am planning to study the localization of GFP-tagged proteins in mycobacterium smegmatis by using confocal microscopy. I need to know to know which fixatives can be used? Would 4% PFA work for...

05 May 2018 6,460 7 View

Can anyone suggest me methods for RNA isolation from mycobacterium species other than bead milling ??

I have used Trizol based liquid nitrogen method but not getting a good yield. I need it for cDNA preparation and qRT-PCR experiments. Any suggestions are welcome. If possible share the protocols....

11 November 2017 6,961 4 View