Renu Kumari

3 Questions 4 Answers 0 Followers

Questions related from Renu Kumari

Restriction digestion and ligation , both should be of same company ?

Can any be from a plant background? i want to know that for digesting the vector and ligating the insert into the digested vector, is it crucial that we use chemicals from the same company as the...

22 March 2024 1,303 1 View

I am doing TA cloning, and i do not get fall out after the restriction digestion. what is its probable reasons?

After transformation in DH5 alpha, I got positive colonies. I have confirmed it by PCR (using an isolated plasmid of positive colonies as a template to run PCR by Takara Taq) and restriction...

15 March 2024 3,672 7 View

For transformation of callus via Agrobacterium what should be optimum OD?

I have transformed at 0.3 OD but callus is not survived in selection media.

04 April 2022 9,302 6 View