Renshan Zhang

6 Questions 5 Answers 0 Followers

Questions related from Renshan Zhang

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

01 August 2024 2,111 0 View

Why basal level of CD69 is so higher in PBMC before IgM stimulation?

Our lab is working on PBMC cell for compound screening. We are surprised by finding that the level of CD69 in PBMC is very high prior to IgM stimulation(50-60% or so), and after IgM stimulation,...

02 June 2024 2,973 2 View

The anti-CD3 in the CD3/CD28 T Cell Activator affect CD3 gating in FACS analysis, how to solve this problem?

I am using ImmunoCult™ Human CD3/CD28 T Cell Activator (Semcell, Catalog #10971) to activate PBMC and expand T cell. but the T cell activator contains an anti-CD3 antibody clone that fully or...

08 May 2024 2,939 3 View

What is the characteristic of expression bacterial cell E. coli WK6?

Hi, I am wondering the difference between bacterial cell E. coli WK6 and other cells, like E. coli (BL21)? There are several cases using the bacterial cell E. coli WK6 to express some...

17 December 2020 7,853 11 View

Why the mutant Arabidopsis with compromised seeds germination rate contains increased amount of insoluble tannin content?

I found from an literature that ft-1 mutant has reduced germination rate, while the insoluble tannin content in the seeds coat is high, so why is that?

09 June 2018 3,275 1 View

Why the surface staining of CD4 and CD8 failed in T cells

Hi, I a work with surface staining of CD4 and CD8, followed by intracellular staining of INFg in T cell stimulated with anti-CD3/28 from PBMC. This is the general protocol I used: 1) Wash the cell...

01 January 1970 1,448 0 View