Rene Christena

5 Questions 5 Answers 0 Followers

Questions related from Rene Christena

What would be the influence of antibiotics on colony morphology?

Based on some observations i found that an imepenem resistant Pseudomonas aeruginosa had different colony morphology from its wild type. So, does this phenomenon occur in other bacteria? If yes...

01 January 2015 8,889 3 View

How can we increase the recovery of DNA from agarose gels?

DNA purification from agarose gels. How can we increase the recovery of DNA from agarose? Even if I use the qaigen purification kit the yield seems to be negligible, around 10-20ng/ul. Any...

05 May 2014 866 7 View

If a supposed antibacterial is dissolved in DMSO, and the initial screening shows a similar zone for DMSO (in case of control), how do we avoid it?

see above

02 February 2014 6,148 1 View

Can anyone help with an ETBR cartwheel assay?

If organisms show fluorescence at very low concentration of etbr in the absence of pump inhibitor what would be the inference? Please suggest a good assay for efflux pump inhibition.

02 February 2014 8,001 0 View

What can be the appropriate annealing temp for PCR if tm of forward primer is 57.9 and that of reverse primer is 60?

Gene amplification.

02 February 2014 2,354 5 View