Razaul Haque

4 Questions 4 Answers 0 Followers

Questions related from Razaul Haque

How to improve cell viability during PBMC isolation?

Dear all, I am isolating PBMC from human blood over Ficoll . I am using 2mM EDTA as a washing buffer. after collecting buffy coat, I washed PBMC 2 times with 2mM EDTA. During washing I set...

06 June 2019 799 5 View

Is there any RNA isolation kit for human PBMC that yield good amount of RNA?

Hello Everyone, I am trying to extract total RNA human PBMC of healthy person. I used TriZole for cell homogenization. I used 2 x 10^6 no cell. But the RNA yield was very low. I got only 494 ng...

11 November 2018 7,555 6 View

how to fix PBMC after stimulation?

Hello everyone, I am Doing Intracellular cytokine staining for IFN-g and IL-17. I want to stimulate cell (Isolated PBMC) with PMA, Ionomycine and Golgi Stop. After 5 h stimulation I want to block...

07 July 2018 4,372 0 View

How to succeed over subcutaneous injection in mouse model?

Dear all, I am trying to develop EAE mouse with MOG peptide & Mycobacterium tuberculosis as an adjuvant & PTX. But I could succeed over it. I think I could not able to administrate the...

05 May 2018 7,251 2 View