Rawda Elshennawy

3 Questions 6 Answers 0 Followers

Questions related from Rawda Elshennawy

Organoids do not grow well after re-plating, what might be the problem?

I am growing ovarian cancer organoids and they grow very well. Sometimes the matrigel is dislodged from the bottom of the plate and I need to replate them. I follow a protocol where I wash the...

07 June 2021 8,683 2 View

How to use amphotericin B in contaminated cell cultures?

I am using ovarian cancer cell lines and they were heavily infected by fungal spores? What is a good conc. Of amphotericin B to use? And how to apply?

30 May 2021 3,131 4 View

Proper Diluent for primary antibody for IHC?

Does anybody know how to choose a proper diluent for my primary antibody? I have a homemade antibody that I am optimising and do not know which diluent to choose between TBS, TBST, PBS?? also I...

04 May 2021 162 6 View