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Questions related from Rawan A El-Madhoun
when I run my PCR reaction I got two product one sharp non specific product ( 300 bp) in addition to the target product which appear very faint 461 bp. the get photo is attached
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My PCR product appear very faint with primer dimer in each samples , I tried to increase annealing temperature , the extension time and the initial denaturation temperature as recommended in PCR...
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I have a PCR product with 260bp size , usually i run it at 2% Agrose electrophoresis and it gave me a clear band as it appear in the lower line of the attached photo. After digestion , the...
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I optimized my PCR Reaction and it gave me very good result , after that some PCR product samples start to appear very faint in comparison with the other samples and the product of the first...
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