Rawan A El-Madhoun

5 Questions 18 Answers 0 Followers

Questions related from Rawan A El-Madhoun

How I can Eliminate sharp non specific product and increase the quantity of the target ?

when I run my PCR reaction I got two product one sharp non specific product ( 300 bp) in addition to the target product which appear very faint 461 bp. the get photo is attached

07 July 2016 2,009 8 View

How can I solve primer dimer & faint PCR product problem ?

My PCR product appear very faint with primer dimer in each samples , I tried to increase annealing temperature , the extension time and the initial denaturation temperature as recommended in PCR...

08 August 2015 5,754 16 View

What is the best Immunology book for beginner ?

Simple immunology book

02 February 2015 3,309 16 View

How can I obtain clear digested PCR product bands in Agrose Gel electrophoresises?

I have a PCR product with 260bp size , usually i run it at 2% Agrose electrophoresis and it gave me a clear band as it appear in the lower line of the attached photo. After digestion , the...

12 December 2014 9,684 22 View

What are the causes of faint PCR product ?

I optimized my PCR Reaction and it gave me very good result , after that some PCR product samples start to appear very faint in comparison with the other samples and the product of the first...

12 December 2014 9,438 14 View