Ravindra Kailasrao Zirmire

6 Questions 21 Answers 0 Followers

Questions related from Ravindra Kailasrao Zirmire

Hi all, Can some one give some suggestion about doing live cell imaging of actin in human fibroblasts by lifeact GFP?

I am trying to visualise actin by lifeact GFP by perkin elmer spinning disc microscope,but for some reason my transfected cells are dying. I have added 100uM HEPES in the media and using...

10 October 2017 6,586 5 View

How can I do live cell imaging of actin dynamics with maximum resolution and maximum time by keeping the actin filaments in focus?

I am very new to the live cell imaging of actin. I am trying to visualise actin filament dynamics by transfecting Lifeact GFP by spinning microscope. I need to visualise the actin filament...

10 October 2017 7,376 5 View

How can we distinguish the normal fibroblast and senescent fibroblast in the culture?

Can we distinguish the normal and senescent fibroblast in culture from their morphology? If during the prcess of trypsinisation if they are not getting trypsinised then can these fibroblast be...

10 October 2014 7,783 16 View

What could be a possible reason if the dermal fibroblasts are not getting detached from the plate even after the trypsinization?

Actually we are looking for the effect of some chemicals on fibroblast and in one of the treatment the newborn dermal fibroblasts are not getting detached from the plate. But in the same...

10 October 2014 1,854 5 View

Does the number of iPSC colony (confluency) affect cardiomyocyte differentiation efficiency?

We are trying to differentiate the Cardiomyocytes from the human iPSC. We could do 6 differentiation sets properly but suddenly our cardiomyocyte differentiation got stoped we think the only...

05 May 2014 3,184 6 View

Can anyone give me an idea about what is the tissue stiffness of the human heart at different developmental stages from foetal to adult stage is?

In a human heart according to the increase in the developmental stage, the heart tissue stiffness (Extra cellular matrix stiffness) goes on increasing. Has anyone checked the human heart tissue...

04 April 2014 8,314 5 View