Ravinder Verma

5 Questions 22 Answers 0 Followers

Questions related from Ravinder Verma

Is there any way we can immunostain a protein in section (paraffin or cryo-section) for which we dont have any reliable antibody ?

My protein of interest doesn't have any reliable antibody and I wanted to immunostain in paraffin, cryo-section or vibrotome section. I am looking for a way to tag the protein in section and...

02 February 2018 9,628 5 View

Are my 293T cells infected?

I have been culturing 293T cells since last 2 years and never had this issue. They develop multiple vesicles seeding after 2-3 days, fail to grow and degenerate. I have tried changing media with...

01 January 2017 8,428 13 View

Disparity in RNAseq and qPCR data ?

We compared control tumor and our gene of interest knock out tumor by RNAseq analysis and got very good comparative results. The problem is when we ran the qPCR using the cell lines derived from...

10 October 2016 3,727 4 View

How many times mouse cortical astrocytes can be passaged ?

I have mouse cortical astrocytes which have already been passaged twice and are in healthy condition but want to passage them again for my experiment. Is it safe to do ? And do I need to use the...

09 September 2016 3,072 5 View

Lentivirus transduce HEK293 cells but not working on murine cell lines ?

Hello everyone! I am working on inducing tumor in mice brain by use of lentivirus. I use 2nd generation packaging plasmids with calcium phosphate transfection method and have very good...

04 April 2016 6,701 21 View