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Hello everyone, I am using Gromacs 2024.2 version of MD simulations and my question is "When performing MD simulations on a protein-ligand complex involving a cofactor and a substrate, is it...
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Hello everyone, As a part of my research work, I am currently working on the identification of intermediates of the enzyme reaction mechanism. I used EPR to analyze the trapped intermediate...
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Hello Everyone, I would like to ask, how to analyze and understand the pre-steady state enzyme kinetics using stopped flow instrument in easy way. Although the instrument (Stopped flow instrument,...
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I want to isolate the membrane proteins from bacteria, I reviewed many articles, they have used 100000 g for separation. I don't have the ultrahigh centrifuge, we have maximum of 10000 g can...
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