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Questions related from Ravi Shankar Gautam
Hi all, I m very much confused for a double digestion reaction to check a fallout of 166 bp which has been cloned in 6900bp vector. I have took around 1500 ng of my recombinant plasmid(6900+166...
27 April 2019 7,409 4 View
I want to ask "does elution buffer provided with kits like gel elution/pcr purification etc interfere with any cloning processes like restriction digestion and ligation? as they contain salt and...
23 November 2018 8,727 9 View
My clone ( cDNA Clone) has 2 bp (C&A) deletion as a result their is frame shift and no protein expression is taking place. gDNA clone reveals no deletion but all my cDNA clone reveals 2bp...
30 July 2018 6,702 8 View
Same cDNA is used for other amplifications and i m getting result for that ,, actually the Tm of the primers is too low unfortunately it is around 35 degree , when i put an PCR at Tm 28 i got some...
08 February 2018 2,879 5 View