Ravi Shankar Gautam

5 Questions 17 Answers 0 Followers

Questions related from Ravi Shankar Gautam

Variable expression of GAPDH and Actin at Rt level in different conditions?

Actually i want to know is it acceptable to get variation in expression of housekeeping genes like GAPDH and Actin which i am getting in Real time pcr? in normal condition actin's avg ct is...

17 July 2019 5,009 1 View

How much concentration of template(Rec Plasmid) required to see a fallout of 166bp?

Hi all, I m very much confused for a double digestion reaction to check a fallout of 166 bp which has been cloned in 6900bp vector. I have took around 1500 ng of my recombinant plasmid(6900+166...

27 April 2019 7,420 4 View

Does elution buffer used for Gel Elution do any interference with restriction digestion/ligation?

I want to ask "does elution buffer provided with kits like gel elution/pcr purification etc interfere with any cloning processes like restriction digestion and ligation? as they contain salt and...

23 November 2018 8,737 9 View

My clone ( from cDNA) after sequencing reveals 2bp deletion in , should I use SDM for repair?

My clone ( cDNA Clone) has 2 bp (C&A) deletion as a result their is frame shift and no protein expression is taking place. gDNA clone reveals no deletion but all my cDNA clone reveals 2bp...

30 July 2018 6,712 8 View

I m trying to clone one gene from cDNA(502bp) and gDNA(600bp), during PCR i m getting amplification from gDNA only not from cDNA, any solution??

Same cDNA is used for other amplifications and i m getting result for that ,, actually the Tm of the primers is too low unfortunately it is around 35 degree , when i put an PCR at Tm 28 i got some...

08 February 2018 2,890 5 View