Raussie Vaid

4 Questions 9 Answers 0 Followers

Questions related from Raussie Vaid

How to create network in cytoscape for deferentially expressed proteins?

I have 74 proteins which have been changed after cell treatment. I would like illustrate a network, based on this genes. I wanted to use cytoscape (v 3.7.1). but I do not know how and in which...

22 September 2020 2,642 3 View

How important it is to add detergent in lysis buffer before protein extraction?

I added it after grinding the tissues in homogenizer. Will it affect the protein extraction process because my protein of interest is missing from the samples.

06 January 2020 9,354 9 View

Why my protein of interest is not consistent in western blot?

Hi, My all samples are from wild type mice and my protein of interest interest is suppose to be present in all samples. But it is present in few and absent in others. All samples are Wildtype but...

31 December 2019 3,838 6 View

Is it necroptosis or apoptosis?

I have perform Hypoxic treatment on AML-12 cells for 12hrs and there is significant up regulation of RIP3 in western blot and no expression of cleaved casp3. I did flow cytometry with PI annexin...

05 June 2018 2,688 4 View