What performance should I have to perform a reverse transcription and how do I improve the A260/A230 ratio since I get values ​​= 0.5?

I'm using the GeneJet RNA extraction kit for plants. I'm following the protocol step by step, but at the end of the extraction I get a yield of 50 ng/uL with a 260/280 >1.85 and 260/230...

5 weeks ago 6,085 1 View