Ratish Raman

4 Questions 13 Answers 0 Followers

Questions related from Ratish Raman

How to screen for different blood cells in the BM of the mice?

Read 8 answers by scientists to the question asked by Ratish Raman on Jun 27, 2017

06 June 2017 9,911 8 View

How will I know if the PCR product is oriented (Fwd. or reverse) in the sequence data?

I have performed cloning for the gene of interest using pcrII TOPO vector. After the slection steps and innoculation steps I isolated the plasmids using QIAGEN kit and then measured using...

09 September 2015 3,250 5 View

How can I improve the yield of PCR purified products using QIAGEN or is there any other method?

I will be procedding with rna probe designing.but before that I am performing pcr to create antisense and sense products and then purifying them. I have performed PCR and using QIAGEN PCR...

09 September 2015 8,711 4 View

Why did LIC for my full length gene and constructs not work?

Hi all I have a full length (FL) sequence gene and few constructs of the gene to produce a functional protein.. which I would purify if all goes well. Firstly, I did normal cloning of my FL gene...

08 August 2014 4,410 5 View