Rao Kv

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Questions related from Rao Kv

Should reducing agents or detergent be used in IB solubilisation and protein purification?

Hi All, I had similar nature of issue with objective of IB solubilisation and about to work between DTT or BME as choice. What would be ideal conc range to work for borh. had you tried native or...

09 September 2014 5,457 6 View

What are the histidine tag removal methods during the purification?

I was cloning a gene with N-terminal Hist tag followed by enterokinase site for cleavage of tag. Is there any method for removal of Hist tag while purification in a single step itself even with...

03 March 2014 2,630 0 View

Can someone advise on availability of codon optimised cDNA Clones for Malaria MSP-1 antigens 19Kda protein?

We are in need of cDNA clones for the Malaria MSP-1 Antigen of Malaria P. Falciparum and P.Vivax for our development works. Vector ligated or cDNA clones of the above MSP-1 protein are of our...

01 January 2014 2,431 2 View