Ranjani Rajasekaran

4 Questions 6 Answers 0 Followers

Questions related from Ranjani Rajasekaran

Will a non-poxviral genome that is placed under a poxviral promoter and between two pox viral genes be expressed by CMV promoter of pCIneo plasmid?

If I construct a recombinant plasmid in the following order of genes in pCIneo plasmid: Fowlpox virus gene 1- early late promoter of poxviral origin - Non-poxviral gene - Fowlpox virus gene...

12 December 2019 449 1 View

Ligated product in LB broth?

I transformed my ligated plasmid onto LB plates with ampicillin. I transferred the colonies to LB broth. After plasmid extraction, I could detect my insert genes with combinations of primers. But...

09 September 2018 7,796 3 View

Can I add more than two restriction sites in my primer?

I want to insert 4 restriction sites between my two genes. Initially, I thought of inserting MCS into my vector and then cloning my two genes on either side of the MCS. But, is it possible to add...

05 May 2017 5,541 6 View

How to identify the replication of NDV in embryonated eggs?

I inoculated NDV containing AAF into 9 day old SPF egg. After 72 hours of incubation, the AAF was harvested. Amplification of M and FPCS gene showed a positive PCR product. But, I am not able...

09 September 2016 4,665 2 View