Ramón Soto

5 Questions 2 Answers 0 Followers

Questions related from Ramón Soto

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

10 December 2018 9,666 3 View

Failure to observe AaRS activity?

Hello all, I work with a tRNA synthetase and I am setting up a classical tRNA aminoacylation reaction with radioactive amino acid. After letting the reaction to run for 1h 30', I precipitated my...

08 June 2016 7,357 11 View

How long can a compound be stable in DMSO for?

I am slightly concerned about my overexpression studies where one of my BCG strains should confer resistance to my control compound, but it seems like either the compound cannot enter the cell or...

26 November 2014 2,574 2 View

How often does M.bovis BCG need to be subcultured?

I am not sure how long I should let my culture grow before doing a subculture. Is it right after log phase (around 1.0 OD) or can it be grown for a longer time? Thanks in advance

30 September 2014 9,358 1 View

Does anyone have experience with gene cloning from M.tuberculosis?

I am reviewing relevant literature for DNA extraction from this bacterium. Considering that my objective is cloning, which is the best protocol to be followed? Which kind of culturing techniques,...

25 February 2014 2,395 3 View