Ramanjot Kaur

5 Questions 5 Answers 0 Followers

Questions related from Ramanjot Kaur

Why i got double bands from gel purified samples?

even after gel purification, I got double bands in some samples. I used the Promega purification kit. I also attach gel pictures with PCR reaction and purified gel product. Please suggest me way...

01 March 2023 3,815 0 View

Why my plasmid concentration on isolation is below 30-70 ng/Microlitre?

As I am using a MACHEREY‑NAGEL nucleon spin plasmid isolation kit. I harvest 3ml from 5ml isolation LB stock which was overnight incubated at 150 rpm. Please suggest ways to improve my quantity.

05 December 2022 5,597 4 View

Why i got different bands sizes for plasmid PCR amplification?

As my desirable insert size is 490 bp but using M13 primer for plasmid amplification I got different band sizes. why there are different types of bands for the same?

01 December 2022 5,463 4 View

How ZFNs (Zinc Finger nucleases) are greatly helpful for targeted genetic manipulations of traits of crop plants?

The emerging genome technologies, including ZFNs (zinc-finger nucleases), TALENs (transcription activator-like effector nucleases), and CRISPR (clustered regularly interspaced short palindromic...

21 April 2022 4,990 1 View

Clones are genotypically identical but not phenotypically?

clones are to assume that they are genetically identical to the organisms being cloned, and will therefore be phenotypically identical to them. kindly elaborate.

10 January 2022 3,588 11 View