Ramachandra Nagaraju

3 Questions 5 Answers 0 Followers

Questions related from Ramachandra Nagaraju

Can i use yeast episomal plasmids to integrate into yeast genome for expression?

I am finding difficult to clone an ion-channel gene in yeast episomal expression vector. All attempts to clone in parts and then subclone to get full length clone also was not successful. So i...

17 May 2016 3,763 7 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

27 January 2016 4,643 2 View

Which nm UV Transilluminator filter should be selected to view Agarose gel with ET Br and SYBR safe ?

I am planning to buy an UV Transilluminator for research work and i am bit confused with the filter nm. Could any one please suggest which nm 254, 302, 312, 365 or combined of 312&365 filter...

31 December 2014 9,771 4 View