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I kept the plasmid in TE buffer in room temperature, assuming plasmids to be stable. But after 2 months I couldn't transform E. coli. Did I do wrong not storing the plasmid in -20 fridge?
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I am expressing a part of a protein with a fusion tag. After cleaving the fusion tag by digestion with protease, the target protein has weight of about 2 kD. Can it be detected by SDS-PAGE? If...
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