Rakesh Bam

5 Questions 8 Answers 0 Followers

Questions related from Rakesh Bam

How can I efficiently isolate individual colonies of cancer cells?

Colonies are >30-50 cells big after two weeks of culture in methylcellulose based media (Methocult), which is semisolid. I tried to capture individual colonies with sharp pipette tips but it...

09 September 2014 1,594 4 View

Just like genecards is helpful for gene analysis, is there a website for human secretomics or protein analysis?

I am analyzing cell culture supernatant proteomics data and trying to exclude proteins that are exclusively cytoplasmic in all human cells since non-secreted proteins might be released by...

07 July 2014 6,388 2 View

How does cell culture density affect gene expression?

Cell-cell contact or lack of contact may alter gene expression and subsequently, cell behavior eg. proliferation. Has anyone done gene expression or cell cycle analysis in relation to cell culture...

12 December 2013 8,768 6 View

How to separate adherent cancer cells grown over MSCs at high purity?

I'm growing cancer cells over MSCs on a flask (these cells adhere to MSCs). At a certain point, I need to extract RNA and protein from these cancer cells. The problem is I need to get RNA or...

02 February 2013 4,717 2 View

RNA extraction using 10k-50k cells?

I have been using RNeasy Micro Kit to extract RNA whenever I have conditions with few cells (example: FACS sorted cells, transiently lentiviral transfected cells). Sometimes I get 100ng/ul RNA in...

08 August 2012 4,644 14 View