13 Questions 26 Answers 0 Followers
Questions related from Rakesh Kumar
I have tested two proteins, they are showing same elipticity pattern in far UV range? at 222nm negative elipticity changes only by 2 degrees, does this indicate something? If not, how one can tell...
19 September 2019 5,604 4 View
Some one please help, I have amplified a 2.3 kb fragment (contains TAP and Kanmx cassete) pYM13 was used as template with overlapping primers. I followed this paper...
13 July 2017 2,882 2 View
Please suggest, How to use colloidal coomassie stained gel for western blotting? Will the dye interfere with transfer? or after transfer if I destain the blot for few second in methanol, will it...
30 April 2017 2,524 1 View
I am working with a protein of approx 35kDa, in SDS PAGE the band is coming at approx 72 kDA. If it is in Dimeric form then why it is not coming as monomeric form. Laoding buffer contains 5% BME,...
26 October 2016 3,031 7 View
I am working with a protein of approx. 45kDa, I want to take spectra in far UV region, protein is highly prone to aggregation so I am getting very low conc. Please suggest what is minimum conc....
14 July 2016 6,791 5 View
I have RNA, isolated from Yeast cells (By TRI (SIGMA) reagent), quality is good after Dnase-1 treatment (on Gel), but cDNA is not formed. MMLV RT is working fine for other strains (RNA samples)....
22 April 2016 3,713 8 View
Please see the attached gel pic and tell me why there are some bands/ smear like appearing in PAGE, Running buffer pH 8.4, loading buffer ph 6.8. I have observed this several times (4-5), the main...
13 October 2015 6,064 7 View
Most often I observe sattelite colonies (small E. coli type colonies) after 8-10 hr of transformation. What are these why they don't grow every time.
11 April 2015 2,050 14 View
What is difference, at the promoter level of these two vectors?
26 February 2015 9,282 1 View
What type of radiation is emmited by (gamma p32) labelled ATP, what could be the effect/ side effect of its expossure,
26 February 2015 2,145 5 View
A Splicing factor Protein that is prone to aggregation at/ above 0.5 mg/ml concentration. It also showed temperature dependent aggregation above 65 C. I need to do some Near UV CD studies, as well...
04 February 2015 6,631 3 View
The protein is in 10 mm buffer and concentration is low (25 micro gram/ml)
08 February 2014 6,362 7 View
I have often noticed red shift after moving from high to low temperature.
08 February 2014 6,835 3 View
