Rajeswari Jayavaradhan

3 Questions 3 Answers 0 Followers

Questions related from Rajeswari Jayavaradhan

Has anyone used miniprep plamids for transfecting HEK 293T cells?

Lipofectamine 3000 transfection of mini prep plasmid (Nucleo Spin kit) into HEK293 T cells. Thanks for any input.

12 December 2015 743 3 View

Why do I get a very low plasmid recovery and quality with the GenElute Endotoxin free plasmid MidiPrep kit?

I have done Mini and maxi preps with macherey nagel kits and had no problems. Tried Sigma GenElute Endotoxin free plasmid Midiprep kit for the first time in DH5alpha cells. Starting culture 50ml,...

12 December 2015 5,721 6 View

Does CD45.1/CD45.2 work on BalbC mice?

Hi, I have IL9R KO mice and I have to check WBC chimerism in BALBc Mice (donor and recipient). I heard CD45.1/CD45.2 doesn't work on BalbC mice. Can you suggest any other marker to distinguish...

12 December 2014 4,244 3 View