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I am using C18 250x4.6 mm. After injecting the column with Poultry feed vitamin premix samples, I noticed a high elevation in the column back pressure. My mobile phase is composed of 99% Methanol...
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Which is the best HPLC column for vitamin analysis? Which column contain highest carbon content?
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When using the Acetonitrile (HPLC grade) (100% Pump B) for long time, there is pressure variation in Pump A & B. Now I solving this problem by ultra sonication of Pumb B filter.
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Now, I am analyzing the vitamins by two methods.
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