Rajan KUMAR Sah

3 Questions 3 Answers 0 Followers

Questions related from Rajan KUMAR Sah

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

05 February 2021 6,921 2 View

How can I troubleshoot my BP reaction?

I am trying BP reaction for gateway cloning for a long time, and getting a lot of colonies. but non of the colonies are positive. steps After adding complete attB1 and attB2 sites to insert,...

22 January 2021 9,454 0 View

How can i get att L and att R sites for the gateway cloning steps?

I want to perform gateway cloning (from LR reaction onwards) , how could i get the LR sequences to attach them with my sequences in donor and insert. Give me your suggestion, i will be thankful...

14 January 2021 3,642 3 View