Raj Kumar

3 Questions 10 Answers 0 Followers

Questions related from Raj Kumar

How can I select the transfected cell to make a stable cell line when cell and plasmid both are G418 (Geneticin) resistant?

Cell and plasmid both are G418 (Geneticin) resistance. So how can i select the transfected cell to make stable cell line. I need to work in same cell line and same plasmid. so any answer is...

02 February 2015 8,143 2 View

How do you dissolve Cisplatin in water?

As the attached document from the company says in water at 25 C, but it does not work. Any ideas? As the solubility of Cisplatin is very low in water how do you make it soluble?

01 January 2015 9,094 8 View

Can someone help me with non specific background fluorescence on GFP mouse kidney tissue stain with 1ary megalin ab and 2ary TRITC ab?

I have stained the GFP mouse kidney with megalin antibody to show the proximal tubule and secondary antibody i used both mouse and rabbit TRITC to check with both of the secondary antibody show...

11 November 2014 6,832 3 View