Rahul Sharma

3 Questions 2 Answers 0 Followers

Questions related from Rahul Sharma

How can we amplify a 3kb gene fragment?

I tried so many times but didn't get anything. It is a tag with the gene located around 66bp on this tag. There is a problem in the case of the C-terminal tag not in being in the N-terminal....

05 May 2014 4,542 8 View

In case of blot washing which one is good PBS or TBS? What is the composition of both solutions?

I used 1X TBST for blot washing but I want to try PBS also please tell me which one good.

10 October 2013 4,064 2 View

I want to know why we use methanol for activation of PVDF membrane before western transfer?

Why do we use nitrocellulose membrane without activation?

09 September 2013 603 12 View