Rahul D. Dani

4 Questions 19 Answers 0 Followers

Questions related from Rahul D. Dani

Do we always need conjugate base salt while preparing buffers? In which case it's needed?

I happened to prepare 1L of 20mM Na-acetate buffer, pH 5. I calculated volume equivalent to 20mM Acetic acid for 1L buffer and adjusted the pH to 5 using NaOH solution. Then I wanted to prepare...

07 July 2017 552 4 View

Protein-ion interaction? Is it direct or indirect?

I have come across some literature regarding Protein-ion interactions. In some of them, the direct mechanism is explained (i.e charge based neutralization, effect of charge density etc.) and in...

06 June 2016 5,260 3 View

How to convert trypsin activity units from BAMC to BAEE?

hi. I want to use Trypsin which is there in our lab. The activity of trypsin is given in terms of BAMC (benzoyl-DL-arginine-7-amido-4-methylcoumarin) as a substrate. The paper I am referring for...

05 May 2016 8,647 1 View

Is perchlorate/ACN mobile phase in HPLC for proteins going to harm their primary structure or is it going to decrease the column life?

There are several papers where people have used perchlorate/ACN combination in HPLC to resolve peptides. However, it is reported that perchlorate in presence of acetonitrile forms free radicals,...

04 April 2016 6,796 1 View