Rahul Singh

6 Questions 9 Answers 0 Followers

Questions related from Rahul Singh

What is the difference between pseudosymmetry and non crystallographic symmetry ?

It is relatively common for protein crystals to crystallize with more than a single molecule per asymmetric unit, which could lead to various pathological conditions. Under such circumstances, is...

13 December 2021 8,281 6 View

Why recombinant expression of GFP in Pichia pastoris (yeast) cannot be confirmed by just visual inspection?

I have cloned a fusion protein cassette with a C terminal GFP tag in E.coli based recombinant protein expression system. The expression of the cloned cassette was confirmed by simple visual...

08 March 2021 1,239 5 View

How to interpret multiple bell shaped curves in Kratky plot?

Hi, I'm trying to analyze a Kratky plot generated from my SAXS data. I am seeing multiple bell-shaped curves in my Kratky plot and I am not able to find any source to get relevant info on this...

21 January 2021 2,683 1 View

Has anyone experienced protein precipitation, just after gel filtration?

I am working on a protein of 25 kDa with pI of 9.29. I have this protein in phosphate buffer 20 mM pH 7, NaCl 150 mM. The protein behave very well during gel filtration (buffer NaAc 40mM pH5.5,...

04 April 2019 6,617 4 View

How to express and purify EcoRV restriction enzymes in E. coil based expression system?

Hello, I am familiar with recombinant expression of protein and downstream processing to purify expressed protein to homogeneity. However, expressing restriction enzyme could be fatal for...

03 December 2016 6,440 3 View

Is my enzyme is showing negative cooperativity?

Dear friends, I am working on Michaelis–Menten kinetics of an enzyme. The enzyme’s molecular weight is 50 KDa, it elutes as a dimer during gel filtration. The Michaelis–Menten saturation curve of...

18 March 2016 2,956 11 View