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Questions related from Rahul Singh
I am a novice in the field of heme-protein interactions. I am investigating a protein that binds heme in its native form. I have also created a mutant version of the protein that is expected to...
01 September 2023 9,848 0 View
It is relatively common for protein crystals to crystallize with more than a single molecule per asymmetric unit, which could lead to various pathological conditions. Under such circumstances, is...
13 December 2021 8,288 6 View
I have cloned a fusion protein cassette with a C terminal GFP tag in E.coli based recombinant protein expression system. The expression of the cloned cassette was confirmed by simple visual...
08 March 2021 1,246 5 View
Hi, I'm trying to analyze a Kratky plot generated from my SAXS data. I am seeing multiple bell-shaped curves in my Kratky plot and I am not able to find any source to get relevant info on this...
21 January 2021 2,716 1 View
I am working on a protein of 25 kDa with pI of 9.29. I have this protein in phosphate buffer 20 mM pH 7, NaCl 150 mM. The protein behave very well during gel filtration (buffer NaAc 40mM pH5.5,...
04 April 2019 6,627 4 View
Hello, I am familiar with recombinant expression of protein and downstream processing to purify expressed protein to homogeneity. However, expressing restriction enzyme could be fatal for...
03 December 2016 6,448 3 View
Dear friends, I am working on Michaelis–Menten kinetics of an enzyme. The enzyme’s molecular weight is 50 KDa, it elutes as a dimer during gel filtration. The Michaelis–Menten saturation curve of...
18 March 2016 2,961 11 View
