Rahul Kumar

6 Questions 3 Answers 0 Followers

Questions related from Rahul Kumar

How to generate or create publication ready maps?

I am trying to generate map to indicate species distribution of a wildlife sanctuary. The sharp boundary of this area is not visible on Google map but vegetation cover and longitude and latitude...

16 May 2021 8,715 8 View

Why did algal blooms which appeared red in the afternoon turn green in the evening?

I need some help with one observation of mine. I observed a pool of water full with algal blooms. The algal blooms of the pond appeared red in the afternoon but in the evening it turned green....

05 April 2021 3,191 4 View

How can I remove Stearylamine/ODA from an organic solution which finally needs to be dissolved in chloroform?

How can I remove Stearylamine/ODA from an organic solution which finally needs to be precipitated and dissolved in chloroform? I am repeatedly washing my solution in acetone by centrifugation and...

07 August 2018 8,865 0 View

How can we detect the cytokines released by cells in the media?

I have antibody against a particular cytokine. I also have that particular cytokine. I want to detect that cytokine if it is released by the cells in the media. How can I prepare the sample using...

15 May 2016 7,427 3 View

Mathematical Conversion of Fold Change Values to Log2 Ratio and Vice Versa in a Microarray Expression Profile, and Generation of Heat Map ???

I have microarray data with fold change values with cut-off value of 0.6 and above for upregulated genes, and -0.6 and below for down regulated genes. I want to ask- how can I convert fold change...

03 May 2016 4,123 8 View

Can we interchangeably use Hank's Balanced Salt Solution and DPBS for washing melanoma cells cultured in RPMI-1640 medium?

Can we interchangeably use DPBS in place of Hank's Balanced Salt Solution (HBSS) for washing melanoma cells cultured in RPMI-1640 medium before trypsinization ? Will pH differences affect the...

21 January 2016 1,698 2 View