Rafiou Agoro

4 Questions 9 Answers 0 Followers

Questions related from Rafiou Agoro

Decrease of CD86 expression in macrophage under LPS stimulation???

Hello everyone! I am surprising to find that bone marrow derived macrophages reduces CD86 expression upon LPS stimulation during 12h. Did anyone find this observation before? Thanks for help!

08 June 2016 1,493 4 View

Which kind of flagelin (TLR5 agonist) can I use to induce strong cytokine response on RAW264.7 macrophages?

Looking for advise about a good flagelin which induces strong macrophage response Briefly, I tried already some flagelin components to induces RAW264.7 response during several time points but I...

10 April 2015 3,348 2 View

How can I extract "only" bacterial RNA from infected liver, lung or spleen?

Hello Everyone, I want to do some mycobacterial RNA isolation from infected organs. I want just the bacterial RNA not host RNA. If you have successfully achieved this please provide me with some...

15 January 2015 341 3 View

How can I increase the purification of my RNA solution after extraction if I found that the ratio 260/230 is very low?

I obtained a good concentration of RNA and a good ratio 260/280 after the measurement by nanodrop. Infortunately I found that the ratio 260/230 was still very low (around the average of...

05 January 2015 9,549 4 View