Rafael Sesmero

5 Questions 13 Answers 0 Followers

Questions related from Rafael Sesmero

Which are the molecular weights of 18S and 28S rRNA in plants?

After a RNA extraction, 1% agarose gel is normally performed to verify the quality/integrity of the RNA. It seems like RNA extraction is fine as far as two bands appear (with no smear),...

04 May 2015 5,686 4 View

How can I get rid of polyphenols in olive leaves RNA extraction?

RNA extractions of olive leaves using trizol method (followed by purification with Phenol: cloroform: isoamylic alcohol 25: 24: 1 and precipitation with AcNa 3M) sometimes yield brownish-greenish...

17 April 2015 8,203 7 View

Does the q-PCR efficiency depend on the cDNA samples?

I have had some preliminary evidences showing different q-PCR efficiencies (HKG and target genes) for different cDNA samples, using the same pair of primers. I reckon that, theoretically,...

30 March 2015 152 6 View

Is there any trace of pesticides or inorganic fertilizer in the fruit, coming from conventional seedlings and grown organically?

Legislation on organic farming in Spain doesn't allow to plant seedlings grown under conventional conditions, nor coming from conventional seeds. I wonder how much of the fungicides, insecticides...

16 July 2014 2,432 12 View

Does anyone have any scientific references for Oidium in cucurbitaceae plants (eg: Cucurbita pepo) and its control with skimmed milk?

Hello everyone, There's a lot of non-scientific information in internet and handbooks dealing with pest control in organic agriculture. I am particularly interested in home-made pest control with...

05 July 2014 2,135 8 View