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Questions related from Rafael Lira
Positively charged/cationic lipids are widely used as drug vehicles by taking advantage of the non-specific electrostatic binding to the negative surfaces of cells. But if cells bothered to make...
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Hi all, It is common sense that cationic amphiphiles are toxic. This is true for cationic detergents, lipids and aggregates such as micelles and liposomes. This is also true com complexes with...
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Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...
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Hi all, I need to measure the fluorescence intensity for a given object (on two different channels) over time. The object moves (drifts) over time, so its position isn't fixed throughout the...
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Hello there, I am trying to coat plastic beads (spherotech beads) with cationic lipid bilayers. There are several protocols for neutral and negatively-charged bilayer formation on polystyrene...
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