Rafael Factor Carandina

5 Questions 9 Answers 0 Followers

Questions related from Rafael Factor Carandina

(Western Blotting/ SDS PAGE / Proteomics) should the sample preparation for studying membrane proteins be different for preparing cytosolic proteins?

I'm trying to find out which membrane protein (~ 30kDa) my samples of mesenchymal stem cells are expressing. I made a SDS-PAGE and the gel was stained with Coomassie Blue. The band in question is...

25 June 2016 8,445 3 View

Can I replace BSA by Human Albumin in western blotting?

Currently I do not have BSA in my lab, but I have Human Albumin.

09 January 2016 9,303 3 View

Are there any tips for avoiding contamination in intestinal cell primary cultures?

I'm cultivating intestinal organoids from Adults rats...but even using antibiotics, the cell culture always contaminates.

18 June 2015 629 4 View

Which antibodies can I use to characterize a Small Intestine Organoid Units cell culture?

I'm making a primary culture cells from small intestine of a rat

14 June 2015 2,955 3 View

Does anyone here work with intestinal cell primary cultures?

Intestinal epithelial organoids

23 August 2013 8,134 10 View