Rafael Factor Carandina

3 Questions 6 Answers 0 Followers

Questions related from Rafael Factor Carandina

(Western Blotting/ SDS PAGE / Proteomics) should the sample preparation for studying membrane proteins be different for preparing cytosolic proteins?

I'm trying to find out which membrane protein (~ 30kDa) my samples of mesenchymal stem cells are expressing. I made a SDS-PAGE and the gel was stained with Coomassie Blue. The band in question is...

25 June 2016 8,427 3 View

Can I replace BSA by Human Albumin in western blotting?

Currently I do not have BSA in my lab, but I have Human Albumin.

09 January 2016 9,297 3 View

Does anyone here work with intestinal cell primary cultures?

Intestinal epithelial organoids

23 August 2013 8,125 10 View