Raed Hmadi

4 Questions 6 Answers 0 Followers

Questions related from Raed Hmadi

What is the best way to get rid of bacterial contamination in a mammalian cell line (mouse embryonic stem cells)?

I have very precious cells (mouse embryonic stem cells) that have been contaminated with bacteria recently. The regular medium I use contains pen/strep, but bacteria is still growing. Is there a...

11 November 2016 3,401 5 View

Anyone succeeded in cloning shRNA into pLKO.3G plasmid?

I am trying to insert a 60 bp shRNA into the pLKO.3G plasmid (variant of pLKO.1), yet nothing is working. I digest with EcoRI and PacI, anneal the oligos at 95 for 4 mins then 70 for 10 mins, then...

09 September 2016 1,718 5 View

Are EGF and FGF stable in culture media at 37 degrees?

For how long will EGF and FGF stay stable in DMEM media at 37 degrees?

12 December 2014 6,570 4 View

What is the best way to plot Rhodamine 123 plots for publications? Should I use flow cytometer histograms?

Rhodamine 123 plots

03 March 2014 4,792 0 View