Rachel Tam

2 Questions 7 Answers 0 Followers

Questions related from Rachel Tam

Has anyone tried to reprogram cancer cells to iPSC?

I am trying to reprogram a mouse liver cancer cell line to iPSC. About 2 weeks after transfection I started seeing mouse ES-like colonies. I dissociated and replated them on new feeders hoping to...

05 May 2016 716 4 View

How to passage cells undergoing iPSC reprogramming?

I am trying to reprogram human and murine cell lines using the 4 times transfection protocol. After the 4th transfection, I plated the cells on a layer of inactivated MEFs. A week has passed since...

01 January 2016 6,050 4 View