Rachana R. Chandran

2 Questions 3 Answers 0 Followers

Questions related from Rachana R. Chandran

Rab-YFP can not be recognized by Anti-YFP antibody but works with Anti-GFP antibody?

Recently we ordered YFP- tagged Rab protein (Rab-YFP) drosophila fly lines (for 15 different Rab proteins) from VDRC. Unfortunately none of them can be recognized by YFP antibody, but can be...

09 September 2014 587 1 View

Any suggestions on companies that do protein purification?

I have hard time purifying a His tagged 15KD protein using bacterial expression system. Looks like there is no problem with the induction, but have trouble getting enough protein. So looking for a...

02 February 2014 4,396 5 View