Rachana L. Patnayak

5 Questions 4 Answers 0 Followers

Questions related from Rachana L. Patnayak

Why are my loading control not even in all the samples?

I am performing western blotting using tissue lysates prepared from white adipose tissues isolated from mice. The targets are membrane proteins. The protein quantification was carried out using...

07 February 2024 8,955 5 View

Why qPCR results shows similar ct values for control and KO mice?

I have nhe6 KO mice, confirmed via genotyping from their tail clips. I isolated BAT, iWAT, and gWAT from three controls and three KO mice. RNA isolation followed by cDNA preparation was performed...

18 October 2023 6,420 3 View

Why are my 3T3L1-mcherrry adipocyte cells getting contaminated?

My 3T3L1-mcherry fibroblast grows perfectly fine, but on differentiation, the cells are getting contaminated. These cells take two weeks to differentiate. For the first week, I grow them in...

24 July 2023 3,371 3 View

How to perform siRNA transfection in hybridoma cells?

I have IgG-secreting hybridoma cell lines, and I am trying to knock out a gene in these cell lines. I tried K/D but, each time I get a very low RNA yield (to assess using qPCR) and K/D is...

16 May 2023 5,699 1 View

What is best way to assess the gene knockdown in hybridoma cells? Is it through qPCR or western blotting?

I am performing gene knockdown in hybridoma cell lines using siRNA transfection.

16 May 2023 6,701 6 View