Rachana L. Patnayak

2 Questions 2 Answers 0 Followers

Questions related from Rachana L. Patnayak

How to perform siRNA transfection in hybridoma cells?

I have IgG-secreting hybridoma cell lines, and I am trying to knock out a gene in these cell lines. I tried K/D but, each time I get a very low RNA yield (to assess using qPCR) and K/D is...

16 May 2023 5,697 1 View

What is best way to assess the gene knockdown in hybridoma cells? Is it through qPCR or western blotting?

I am performing gene knockdown in hybridoma cell lines using siRNA transfection.

16 May 2023 6,643 6 View