Rachael Dooley

4 Questions 15 Answers 0 Followers

Questions related from Rachael Dooley

Can anyone suggest some good hormone pulse detection software?

I am trying to detect gonadotropin-releasing hormone pulses for my research. I am having trouble finding suitable software for this. Does anyone have any suggestions on how or where I could find this?

05 April 2016 7,060 12 View

Should I ligate my two DNA fragments together first and then ligate that construct into my plasmid? Or throw all three together in at the same time?

I have two fragments one is 2.9kb the other is 2.7kb. I need to ligate those two fragments together and then ligate my new construct into a 4.36kb plasmid. Should I do two separate reactions, one...

17 March 2015 8,824 13 View

Do I need gel purification after digestion for plasmid ligation?

I have just recently digested both of my amplicons (with bamh1 and bgl1) and my plasmid (with bamh1), in all of them I heat inactivated them. Do I really need to gel purify before I do my...

12 March 2015 283 18 View

Is there a SV40 large T-antigen encoding region for sale already ligated into a plasma that I could purchase somewhere?

I am trying to create a construct consisting of the GnRH promoter region from a chicken and the SV40 large t-antigen (TAg) encoding region for my master's thesis (to later create an immortalized...

06 March 2015 2,949 2 View