R. Alan Burgess

2 Questions 2 Answers 0 Followers

Questions related from R. Alan Burgess

Why would the m/z match the neutral mass in positive MALDI qTOF?

I’m working with steroids, androgens specifically, and I have 2 compounds that give me m/z’s that match the neutral mass. The 2 compounds are dihydrotestosterone and androstanedione. I’ve tried...

31 October 2024 7,476 0 View

How much larger can my fragment be, compared to my target, in homologous recombination?

I'm looking to use a lambda red system to do homologous recombination (Datsenko and Wanner, PNAS 2000) in Shigella flexneri. Specifically, I want to incorporate a Kanamycin resistance gene into...

13 February 2017 1,863 4 View