Quyen Nguyen Thi

4 Questions 3 Answers 0 Followers

Questions related from Quyen Nguyen Thi

Could you suggest ways to improve the strength of a generated monoclonal antibody? Can we clone mAbs from infected host animals such as cows,.. ?

Dear everyone, We generated mAbs via hybridoma systems. The mAbs show high antigen binding affinity and specificty. However I still wonder what is the best way improve the strength of the mAb? To...

27 November 2019 6,007 3 View

Could I tract Alum in Dendritic cell using Lumogallion?

Does anyone have experience with tracking Aluminum salt in Dendritic cells? I found that alum can be tracted in viable cell by lumogallion staining. But all papers I found they used THP-1 cells...

20 June 2017 3,659 0 View

What is positive control for memory B cell ELISPOT assay?

Dear all, I'm going to quantify memory B cells using ELISPOT assay. I will use splenocytes from immunized C57BL/6 mice. Could you recommend any possitive control for this? Thank you very much!

16 February 2016 9,573 3 View

Dendritic cell activation and maturation markers?

Dear all! I'm checking mouse DC activation and maturation . I did expression of CD40, CD80, CD86 and MHC-II For cytokine production, I measure IL12, IL1-beta, and TNF-alpha. I would like to check...

27 January 2016 4,646 5 View