Quang Nhat Nguyen

9 Questions 12 Answers 0 Followers

Questions related from Quang Nhat Nguyen

How to combine scRNASeq datasets for pooled Seurat analysis of individual donors and combined donors?

Hi everyone, From 3 donors, I isolated 2 cell populations (Xneg and Xpos) from blood and tissue. They are then sequenced separately, giving me 4 10X Cell Ranger datasets (matrix.mtx, genes.tsv,...

10 January 2021 2,681 0 View

What are the sequences for HIV LTR-CAT plasmid?

Hi all - Anyone has the nucleotide sequences for either one of the following plasmids? These are very standard plasmids, but I could not find their sequences online. Thanks in advance! HIV...

08 March 2017 4,123 0 View

How to best represent amino acid alignment data?

Hi everyone - I have a large dataset of aligned single-genome analysis (SGA) amino acid sequences from multiple species, tissues, and timepoints. I would like to visualize the differences among...

12 January 2017 7,164 3 View

How can we predict Protein Interactions based on DNA/RNA Sequences?

If RNA or DNA sequences, e.g. metagenome of a sample are given, is there a software or technology allows us to construct/translate "pseudo-proteins" from these sequences? If so, would you mind...

11 February 2016 4,518 3 View

Does anyone have any suggestion for a monoclonal antibody that does not bind at all to bacterial ATP Synthase subunit beta?

I am looking for a negative control mAb that does not bind to bacterial ATP synthase subunit beta protein in Western blot under denature and/or native conditions. I have tried 7b2, synagis, CH65,...

18 August 2015 6,524 3 View

Why do we perform Reverse transcription PCR to amplify immunoglobulin genes from B cells and not the DNA directly?

I'm getting trained on performing B cell PCR; however, I understand that DNA-RNA-protein and if our goal is to produce the antibody, then it may make sense to amplify RNA directly, but do we have...

31 July 2015 2,162 6 View

How to prevent Coomassie R-250 Stain remaining after transferring onto PDVF membrane (before primary incubation) in Blue Native PAGE Western Blot?

I attempted to run: Goal: To test binding between a protein in its native conformation and a monoclonal antibody that shows cross-reactivity with this protein in its denatured form (determined by...

28 July 2015 589 9 View

Why would one want to flow-based sort plasmablast instead of memory B cells to study antibody response?

I heard that researchers begin to sort on plasmablast instead of memory B cells, especially in studies of antibody response. Would someone with experience in Flow Cytometry staining/sorting in...

16 June 2015 5,586 5 View

How can I identify which protein(s) an antibody may bind in a stool lysate?

Hello everyone, For my summer project, I am trying to see if a monoclonal antibody binds to any protein in a stool lysate. Initially, I tried to run them in a regular denaturing gel, and blotted...

11 June 2015 4,349 2 View