Quang Nhat Nguyen

6 Questions 9 Answers 0 Followers

Questions related from Quang Nhat Nguyen

How to combine scRNASeq datasets for pooled Seurat analysis of individual donors and combined donors?

Hi everyone, From 3 donors, I isolated 2 cell populations (Xneg and Xpos) from blood and tissue. They are then sequenced separately, giving me 4 10X Cell Ranger datasets (matrix.mtx, genes.tsv,...

10 January 2021 2,672 0 View

How to best represent amino acid alignment data?

Hi everyone - I have a large dataset of aligned single-genome analysis (SGA) amino acid sequences from multiple species, tissues, and timepoints. I would like to visualize the differences among...

12 January 2017 7,151 3 View

How can we predict Protein Interactions based on DNA/RNA Sequences?

If RNA or DNA sequences, e.g. metagenome of a sample are given, is there a software or technology allows us to construct/translate "pseudo-proteins" from these sequences? If so, would you mind...

11 February 2016 4,513 3 View

Does anyone have any suggestion for a monoclonal antibody that does not bind at all to bacterial ATP Synthase subunit beta?

I am looking for a negative control mAb that does not bind to bacterial ATP synthase subunit beta protein in Western blot under denature and/or native conditions. I have tried 7b2, synagis, CH65,...

18 August 2015 6,519 3 View

How to prevent Coomassie R-250 Stain remaining after transferring onto PDVF membrane (before primary incubation) in Blue Native PAGE Western Blot?

I attempted to run: Goal: To test binding between a protein in its native conformation and a monoclonal antibody that shows cross-reactivity with this protein in its denatured form (determined by...

28 July 2015 586 9 View

How can I identify which protein(s) an antibody may bind in a stool lysate?

Hello everyone, For my summer project, I am trying to see if a monoclonal antibody binds to any protein in a stool lysate. Initially, I tried to run them in a regular denaturing gel, and blotted...

11 June 2015 4,344 2 View