Qiao Gan

3 Questions 10 Answers 0 Followers

Questions related from Qiao Gan

The wrong size of protein that I want to overexpression?

I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...

06 November 2017 1,365 3 View

We purified a protein with yellowish brown color

We purified a protein with yellowish brown color From Ni-NTA Column, but I don't know why. The protein with 70 amino acid contains 12 cysteines. And I want to seek the protocol that how to...

28 July 2017 4,478 11 View

Could cells uptake glucose trigger some key factor might induce cells death?

I am a little confuse what the condition of cells when I add a kind of protein the cancer cell uptake glucose, but the cells upregulate the apoptosis associated protein and the capacity of...

03 January 2017 6,893 5 View