4 Questions 32 Answers 0 Followers
Questions related from Pui-Mun Chan
What is the optimal protein concentration of cell lysate used for this assay kit?
08 September 2014 3,099 2 View
I've done RT-PCR and found that the macrophage (from mouse) strain that I'm dealing with does not have NF-kB gene. However, there is IL-6, iNOS, and TNF-alpha. So, is it possible that IL-6 and...
13 June 2013 6,922 17 View
What are the possible inflammatory pathways that induce the production of TNF-alpha and IL-6 by LPS without going through NF-kB pathway?
11 June 2013 1,771 1 View
I tried to activate NF-kB in RAW264.7 cells using 1 microgram/mL of LPS and check for the expression using an ELISA kit. However, I couldn't get the results. The kit is absolutely fine because...
16 April 2013 5,626 42 View