Prranshu Yadav

6 Questions 5 Answers 0 Followers

Questions related from Prranshu Yadav

Can someone please explain why I am getting this irregular and wavey band pattern in SDS-PAGE?

The sample I loaded is yeast cell lysate lysed by sonication.

15 November 2016 4,599 3 View

I need to isolate intact polysomes from yeast cells using ultracentrifugation. Are there any chemicals that lyse cells without disrupting polysomes?

Chemicals like DMSO and non denaturing detergents like triton x-100 come to mind. But I am not sure if they will spare the polysomes. My cells seem to be too hardy to be lysed by glass beads...

11 January 2016 9,694 3 View

How can I get rid of a LYS2 marked plasmid in yeast?

I have a yeast strain containing a LYS2 marked plasmid, which makes it prototrophic for lysine. Without the plasmid, it would be auxotrophic for lysine. I would like to know if there is any method...

06 July 2014 7,704 2 View

Is it possible to isolate plasmids from a contaminated E coli culture?

My E coli culture has started showing some clumps after repeated innoculations and I suspect it is contaminated. I do not have another culture containing my plasmid.

22 December 2012 9,130 9 View

Does anyone know of a paper that shows the relative abundance of E3 ligase, GPCR and protein kinase genes.

I need a reference to substantiate the fact that E3 ligase genes make up a greater part of genome than GPCR or protein kinase genes. Even a review just mentioning this will suffice.

01 December 2012 658 0 View

Can development of liposome based drug delivery vehicles be made easier using mathematical optimization techniques?

This is just something I have been pondering over for a while and would like to discuss with others. Its not exactly my area of research right now, so I would like to know from those in the field...

01 January 1970 9,004 4 View