Priyanka Siwach

6 Questions 7 Answers 0 Followers

Questions related from Priyanka Siwach

How to use EST assembly for a annotated genome for finding some relevant information ?

i started working on est assembly and annotation of a plant species some months back, till that time complete genome sequence was not available. Now the species has its genome sequenced and...

04 October 2016 5,288 3 View

How to analyse the miranda 3.3a output and to check cross species conservation status of predicted targets?

we have used miranda 3.3a for target prediction for our novel miRNA dataset obtained from NGS data. We have obtained the output. This is a very big file and difficult to analyze manually. Further...

04 May 2016 380 0 View

What is the effect of genotype pedigrees on association mapping of traits?

I have selected 95 genotypes for association mapping of quality traits using 500 markers. Five of the genotypes have one parent in common. How will this effect the association mapping?

13 May 2014 4,175 1 View

Can anyone help with cleaning of EST sequences?

I have started working on EST data processing of cotton. As a first step, we have screened all the deposited EST sequences by 'VecScreen' to detect vector sequence contamination. But in all the...

12 May 2014 7,197 3 View

How can I trim the terminal oligonucleotide from EST sequences of a large datasets? (nearly 60,000 sequences to be trimmed)

I am working on an EST data assembly and annotation project. After screening with VecScreen no significant matches were detected. As a cautious step, we plan to trim the terminal 25-30 nt length...

12 May 2014 463 3 View

How to calculate PIC, MI and H' values for ISSR primers?

We are working with SSR and ISSR primers. The resolving power of various SSR primers to distinguish among the genotypes could be evaluated by calculating PIC, Marker index, Shanon index. We used...

10 January 2014 5,572 7 View