Priyanka Siwach

3 Questions 5 Answers 0 Followers

Questions related from Priyanka Siwach

Can anyone help with cleaning of EST sequences?

I have started working on EST data processing of cotton. As a first step, we have screened all the deposited EST sequences by 'VecScreen' to detect vector sequence contamination. But in all the...

12 May 2014 7,185 3 View

How can I trim the terminal oligonucleotide from EST sequences of a large datasets? (nearly 60,000 sequences to be trimmed)

I am working on an EST data assembly and annotation project. After screening with VecScreen no significant matches were detected. As a cautious step, we plan to trim the terminal 25-30 nt length...

12 May 2014 445 3 View

How to calculate PIC, MI and H' values for ISSR primers?

We are working with SSR and ISSR primers. The resolving power of various SSR primers to distinguish among the genotypes could be evaluated by calculating PIC, Marker index, Shanon index. We used...

10 January 2014 5,558 7 View